The Naica System leverages the key assets of Crystal Digital PCR in a compact, easy-to-use, fast and reliable digital PCR solution.
A single consumable per experiment
Two instruments for the entire workflow
Digital PCR results in under 2 hours, including thermocycling
Less than 5 min hands-on time
A unique 3-color detection
User-friendly analysis software
Crystal Digital PCR is Stilla’s next-generation technology for absolute quantification of nucleic acids.
Using cutting-edge microfluidic innovations, this technology integrates the digital PCR process in a single consumable. The sample is first flowed through a network of microchannels and partitioned into a large 2D array of 25,000 to 30,000 individual droplets, also called a droplet crystal. PCR is then performed on-chip and the crystal is imaged to reveal the droplets that contain amplified targets. The last step consists in counting the number of these positive droplets to precisely extract the absolute quantity of nucleic acids.
With Crystal Digital PCR, the combination of powerful image analysis and intuitive visual inspection offers an unmatched level of confidence in the digital PCR measurement, yielding data you can truly trust.
Load the reaction mixes into the wells of the Sapphire chips
Place the chips into the Naica Geode
Launch the Crystal Digital PCR program
Crystals of 25,000 to 30,000 droplets are created from each sample
PCR amplification is performed immediately after crystal generation
Transfer the chips to the Naica Prism3 crystal reader
Measure the concentrations of targeted nucleic acids with Crystal Miner software
Crystals are read using 3 fluorescent channels
1, Mail du Professeur Georges Mathé, 94800 Villejuif, FRANCE
Tel : (+33) 09 82 29 50 50